Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: HVMLOH1A

[Submit comment/correction]

Locus	HVMLOH1A
Type	Microsatellite
Chromosome	4H
Map	Barley-ConsensusFHB-4H 110.43 Hordeum-Consensus2006-DArT-4H 120.1 Hordeum-Consensus2007-SSR-4H 102.27 Hordeum-FxS-FHB-QTL-4H 189.4 Hordeum-LxHs-4H 88
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2006, DArT Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, FxS, FHB QTL Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	HVMLOH1A
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206. Mesfin A et al. (2003) Quantitative trait loci for fusarium head blight resistance in barley detected in a two-rowed by six-rowed population Crop Science 43:307-318. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Number of populations contributing to the placement of HvMloh1A in the Barley, Consensus 2006 DArT maps = 2 Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (GA)6 Polymorphic Information Content (PIC) = 0.52 Developing laboratory = SCRI (R. Waugh)
Probe	HVMLOH1A
Locus	HVMLOH0001A HVMLOH1A
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (GA)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.52
Type	PCR SSR
PCR primers	5' CCTCCCCTCTGATATGATAA 3' 5' GTACAGACGGTTTAATTGTCC 3' CCTCCCCTCTGATATGATAA GTACAGACGGTTTAATTGTCC Forward CCTCCCCTCTGATATGATAA Reverse GTACAGACGGTTTAATTGTCC
SSR size	175
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare