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GrainGenes Marker Report: HVSS0001

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Locus	HVSS0001
Type	Microsatellite
Map	Barley, B73xCPI-7H 71.4
	[ Show Nearby Loci ]
Map Data	Barley, B73xCPI
Probe	HVSS0001 HVSS1
Probe for Locus	HVSS1
Locus	HVSS0001 HvSS1
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks	Insert contains repeat (t)20
Type	SSR
PCR primers	Forward TGGCCTTGGTGAATGTTTCG Reverse TGTGGAGGCGAGTCAGCTTG Left 5' TGGCCTTGGTGAATGTTTCG Right 5' TGTGGAGGCGAGTCAGCTTG TGGCCTTGGTGAATGTTTCG TGTGGAGGCGAGTCAGCTTG
SSR size	240
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Source Species	Hordeum vulgare