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GrainGenes Marker Report: HVTUB

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Locus	HVTUB
Type	Gene Microsatellite
Synonym	Synonym HVTUB
Chromosome	2H 4H
Map	Barley, B73xCPI-1H 132.5 [ Show all 11 ]
	[ Show Nearby Loci ]
Map Data	Barley, Abiotic QTL Consensus [ Show all 8 ]
Species	Hordeum spontaneum Hordeum vulgare
Probe	HVTUB
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Rostoks N et al. (2005) Genome-wide SNP discovery and linkage analysis in barley based on genes responsive to abiotic stress. Molecular Genetics and Genomics 274:515-527. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AGC)8 Polymorphic Information Content (PIC) = 0.1 Developing laboratory = SCRI (R. Waugh)
Probe	HVTUB
Locus	HVTUB
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (AGC)8 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.1
Type	PCR SSR
PCR primers	5' CATTCTTTACACCCAAGAAAG 3' 5' CAGTAGAGCTCCCAGCAA 3' CATTCTTTACACCCAAGAAAG CAGTAGAGCTCCCAGCAA Forward CATTCTTTACACCCAAGAAAG Reverse CAGTAGAGCTCCCAGCAA
SSR size	149
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Sequence	X99623
Source Species	Hordeum vulgare