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GrainGenes Marker Report: Hv13GEIII

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Locus	Hv13GEIII
Type	Microsatellite
Chromosome	3H
Map	Hordeum-Pillen-3H 3
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Map Data	Barley, AxHs/QTL
Probe	Hv13GEIII
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	Hv13GEIII
Locus	Hv0013GEIII Hv13GEIII
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (at)5 Polymorphic Information Content (PIC) = 0.35 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	GAGGAACCCTACGCCTTACGAG AGGACCGAGAGTGGTGGTGG Left 5' GAGGAACCCTACGCCTTACGAG Right 5' AGGACCGAGAGTGGTGGTGG
SSR size	175
Amplification Conditions	High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	X67099
Source Species	Hordeum vulgare