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GrainGenes Marker Report: Hv52867

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Locus	Hv52867
Type	Microsatellite
Chromosome	4H
Map	Hordeum-Lx41-4H 87.2
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Map Data	Barley, Lx41
Probe	Hv52867
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	Hv52867
Locus	Hv52867
Synonym	Synonym HAR35_44735
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (gt)4 Polymorphic Information Content (PIC) = 0.33 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	Forward GATGAAGGCGCGTCGAATG Reverse CCAACTGCTCGCCAACACAC Left 5' GATGAAGGCGCGTCGAATG Right 5' CCAACTGCTCGCCAACACAC
SSR size	235
Amplification Conditions	High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	U52867
Source Species	Hordeum vulgare