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GrainGenes Marker Report: Hv5sB

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Locus	Hv5sB
Type	Microsatellite
Map	Hv-Integrated2009-3H 76.5
	[ Show Nearby Loci ]
Map Data	Barley, Integrated, Marcel 2009
Probe	Hv5s
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L94 x Vada
Probe for Locus	Hv5s
Locus	Hv5s Hv5sA Hv5sB
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AGT)5 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' CCTCATTTACTTATTTTTGACG 3' 5' AATGCAACACGAGGACTT 3' CCTCATTTACTTATTTTTGACG AATGCAACACGAGGACTT
SSR size	238
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Sequence	Z11436
Source Species	Hordeum vulgare