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GrainGenes Marker Report: HvACL3
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Locus
HvACL3

Type
Microsatellite

Chromosome
7H

Map
Hordeum-Consensus2006-DArT-7H 87.4
Hordeum-Graner2-7H 120.8
Hordeum-Lx41-7H 83.9

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Map Data
Barley, Consensus 2006, DArT
Barley, IxF
Barley, Lx41

Probe
HvACL3

Reference

Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.

Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.

Remark
Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Number of populations contributing to the placement of HvACL3 in the Barley, Consensus 2006 DArT maps = 1

Probe
HvACL3

Locus
HvACL3

Reference

Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.

Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.

Remarks
Insert contains repeat (t)10
Polymorphic Information Content (PIC) = 0.36 in 28 mainly German barley cultivars and two H. spontaneum accessions.

Type
SSR

PCR primers
Forward CCGTCTAGTAGGAGGAGTTATGG
Reverse CAAATCTTGTTCTCGCGGTCG
Left 5' CCGTCTAGTAGGAGGAGTTATGG
Right 5' CAAATCTTGTTCTCGCGGTCG

SSR size
204

Amplification Conditions
High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C

Source Species
Hordeum vulgare