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GrainGenes Marker Report: HvAQ

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Locus	HvAQ
Chromosome	6H
Probe	HvAQ
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	HvAQ
Locus	HvAQ
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (ttc)4
Type	SSR
PCR primers	Forward GAACCCTCTGCCCCTCGC Reverse CGCTTTGCTATGTCGGTCCC Left 5' GAACCCTCTGCCCCTCGC Right 5' CGCTTTGCTATGTCGGTCCC
SSR size	206
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	L43971
Source Species	Hordeum vulgare