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GrainGenes Marker Report: HvBKIN12G

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Locus	HvBKIN12G
Chromosome	3H
Probe	HvBKIN12G
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	HvBKIN12G
Locus	HvBKIN12G
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (t)8
Type	SSR
PCR primers	Left 5' GGCTGCATGGTATTGGTTGG Right 5' GGTTGGTAAAGAACGCCCCAC
SSR size	205
Amplification Conditions	High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	X65606
Source Species	Hordeum vulgare