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GrainGenes Marker Report: HvBTAI0003

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Locus	HvBTAI0003
Type	Microsatellite
Map	Barley, B73xCPI-4H 105.1
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Map Data	Barley, B73xCPI
Probe	HvBTAI0003 HvBTAI3
Probe for Locus	HvBTAI3
Locus	HvBTAI0003 HvBTAI3
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks	Insert contains repeat (gcc)4 Polymorphic Information Content (PIC) = 0.35 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	Forward GCCGCAGTCTCCTCCTCTTG Reverse ACTTGGCGAGGTATGGCTGC GCCGCAGTCTCCTCCTCTTG ACTTGGCGAGGTATGGCTGC Left 5' GCCGCAGTCTCCTCCTCTTG Right 5' ACTTGGCGAGGTATGGCTGC
SSR size	234
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Source Species	Hordeum vulgare