Query (optional) in Class
GrainGenes Marker Report: HvGLUEND
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Locus
HvGLUEND

Type
Microsatellite

Chromosome
1H
2H

Chromosome Arm
1HL

Map
Barley, B73xCPI-2H 64.9
Hordeum-Consensus2006-DArT-2H 72.3

[ Show Nearby Loci ]

Map Data
Barley, B73xCPI
Barley, Consensus 2006, DArT

Probe
HvGLUEND

Reference

Wenzl P et al. (2006) A high-density consensus map of barley linking DArT markers to SSR, RFLP and STS loci and agricultural traits BMC Genomics 7:206.

Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.

Remark
Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Number of populations contributing to the placement of HvGLUEND in the Barley, Consensus 2006 DArT maps = 1

Probe
HvGLUEND

Locus
HvGLUEND

Reference

Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80.

Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.

Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.

Remarks
Insert contains repeat (t)11

Type
SSR

PCR primers
Forward TTCGCCTCCATCCCACAAAG
Reverse GCAGAACGAAAGCGACATGC
Left 5' TTCGCCTCCATCCCACAAAG
Right 5' GCAGAACGAAAGCGACATGC
TTCGCCTCCATCCCACAAAG
GCAGAACGAAAGCGACATGC

SSR size
262

Amplification Conditions
High Stringency: 10 cycles of 1 min at 94 deg C; 0.5 min starting at 64 deg. C and decreasing 1deg C per cycle, 1 min at 72 deg C, followed by 30 cycles of 1 min at 94 deg C; 1 min at 55 deg C; 1 min at 72 deg C; and a final extension step of 5 min at 72 deg C

Sequence
X56260

Source Species
Hordeum vulgare