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GrainGenes Marker Report: HvHEMH1

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Locus	HvHEMH1
Type	Microsatellite
Chromosome	5H
Map	Hordeum-Graner2-5H 43.8 Hordeum-Lx41-5H 0
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Map Data	Barley, IxF Barley, Lx41
Probe	HvHEMH1
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	HvHEMH1
Locus	HvHEMH1
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (t)8 Polymorphic Information Content (PIC) = 0.44 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	Forward TCCTGGCCTTCTTCCTGCTG Reverse AATTATATTGTTATTATCATGGCAGTC Left 5' TCCTGGCCTTCTTCCTGCTG Right 5' AATTATATTGTTATTATCATGGCAGTC
SSR size	194
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Source Species	Hordeum vulgare