GrainGenes Marker Report: HvID
Locus
HvID
Type
Microsatellite
Chromosome
7H
Map
Barley-ConsensusFHB-7H 164.77 Hordeum-Consensus2007-SSR-7H 157.12 Hordeum-LiHs-SNP-7H 208.04 Hordeum-LxHs-7H 182
[ Show Nearby Loci ]
Map Data
Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, Lina x H.spont, SNP Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
HvID
Reference
Remark
Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (AC)16(AT)10 Developing laboratory = SCRI (R. Waugh)
Probe
HvID
Locus
HvID
Reference
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks
Insert contains repeat (AC)16(AT)10 Quality = 2: Single product, relatively strong stutter bands Null in H. spontaneum Canada Park.
Type
PCR SSR
PCR primers
5' GACATTTTTTATAAATTAAGAGCG 3'
5' ATTAACAATCTGCATTAATTGTG 3' GACATTTTTTATAAATTAAGAGCG
ATTAACAATCTGCATTAATTGTG
SSR size
182
Amplification Conditions
1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Sequence
D10057
Source Species
Hordeum vulgare
GrainGenes Marker Report: HvID
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