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GrainGenes Marker Report: HvLOXC

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Locus	HvLOXC
Type	Microsatellite
Chromosome	5H
Map	Barley, B73xCPI-5H 111.8 Barley-consensus-2003-5H 93.1 Hordeum-Lx41-5H 67
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Map Data	Barley consensus 2003 Barley, B73xCPI Barley, Lx41
Probe	HvLOXC
Reference	Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Data Source	Langridge, Peter J.
Remark	Assigned to a barley chromosome by PCR screening of wheat barley addition lines
Probe	HvLOXC
Locus	HvLOXC
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
Remarks	Insert contains repeat (tg)8 Polymorphic Information Content (PIC) = 0.19 in 28 mainly German barley cultivars and two H. spontaneum accessions.
Type	SSR
PCR primers	CAAACACCTCCGACCACACG CATGCACCGGCACAACTCTC Forward CAAACACCTCCGACCACACG Reverse CATGCACCGGCACAACTCTC Left 5' CAAACACCTCCGACCACACG Right 5' CATGCACCGGCACAACTCTC
SSR size	182
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	L37358
Source Species	Hordeum vulgare