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GrainGenes Marker Report: HvMYB1

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Locus	HvMYB1
Type	Microsatellite
Map	Hv-Integrated2009-5H 100.4 Hv-Nure/Tremois-5H 99.8
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Map Data	Barley, Integrated, Marcel 2009 Barley, Nure x Tremois
Probe	HvMYB1G
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Nure x Tremois
Probe for Locus	HvMYB1G
Locus	HvMYB1G HvMYB1
Reference	Hayden MJ et al. (2008) Multiplex-Ready PCR: A new method for multiplexed SSR and SNP genotyping BMC Genomics 9:80. Pillen K et al. (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars Theoretical and Applied Genetics 101:652-660.
General Remarks	Insert contains repeat (ttc)4
Type	SSR
PCR primers	Forward CCCCTGTCTGTGACGTGGAG Reverse CAACGGGGCAAACAAAATGG Left 5' CCCCTGTCTGTGACGTGGAG Right 5' CAACGGGGCAAACAAAATGG
SSR size	176
Amplification Conditions	Low Stringency: 35 cycles of 1 min at 94 deg C; 1 min at 50 deg C; 2 min at 72 deg C; and a final extension step of 5 min at 72 deg C
Sequence	X70879
Source Species	Hordeum vulgare