GrainGenes Marker Report: HvWaxy4a
Locus
HvWaxy4a
Type
Microsatellite
Chromosome
7H
Map
Barley-consensus-2003-7H 24.3 Hordeum-Consensus2007-SSR-7H 23.21 Hordeum-LxHs-7H 27 Hv-Consensus2006-Marcel-7H 23.21 Hv-Integrated2009-7H 25.5
[ Show Nearby Loci ]
Map Data
Barley consensus 2003 Barley, Consensus 2006, Marcel Barley, Consensus 2007, SSR Barley, Integrated, Marcel 2009 Barley, LxHs
Species
Hordeum spontaneum Hordeum vulgare
Probe
HvWaxy4
Reference
Data Source
Langridge, Peter J.
Remark
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
[ Show all 6 ]
Possible Orthologs
HvWaxy
[ Show all 10 ]
Probe for Locus
HvWaxy4
Locus
HvWaxy4 HvWaxy4b HvWaxy4a HvWaxy
Reference
Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391.
Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks
Insert contains repeat (GCAT)4 Quality = 2: Single product, relatively strong stutter bands
Type
PCR SSR
PCR primers
(GCAT)4GBM1326
PCR_primers (GCAT)4Hv49505
PCR_primers 5' AGTATCGCAGACGCTCAC 3'
5' GTTATGTACTCGCTCGCTC 3' AGTATCGCAGACGCTCAC
GTTATGTACTCGCTCGCTC
SSR size
169
Amplification Conditions
SSR_size 1 cycle of 3 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 53C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species
Hordeum vulgare
GrainGenes Marker Report: HvWaxy4a
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