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GrainGenes Marker Report: HvWaxy4b

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Locus	HvWaxy4b
Type	Microsatellite
Chromosome	6H
Map	Barley-ConsensusFHB-6H 63 Hordeum-Consensus2007-SSR-6H 64.71 Hordeum-LxHs-6H 104
	[ Show Nearby Loci ]
Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	HvWaxy4
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (GCAT)4 Developing laboratory = SCRI (R. Waugh)
Possible Orthologs	HvWaxy [ Show all 10 ]
Probe for Locus	HvWaxy4
Locus	HvWaxy4 HvWaxy4b HvWaxy4a HvWaxy
Reference	Hearnden PR et al. (2007) A genetic map of 1,000 SSR and DArT markers in a wide barley cross. Theoretical and Applied Genetics 115:383-391. Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (GCAT)4 Quality = 2: Single product, relatively strong stutter bands
Type	PCR SSR
PCR primers	(GCAT)4GBM1326 PCR_primers (GCAT)4Hv49505 PCR_primers 5' AGTATCGCAGACGCTCAC 3' 5' GTTATGTACTCGCTCGCTC 3' AGTATCGCAGACGCTCAC GTTATGTACTCGCTCGCTC
SSR size	169
Amplification Conditions	SSR_size 1 cycle of 3 min @ 94C, 1 min @ 53C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 53C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare