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GrainGenes Marker Report: HvXan

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Locus	HvXan
Type	Microsatellite
Chromosome	2H
Map	Barley-ConsensusFHB-2H 69.37 Hordeum-Consensus2007-SSR-2H 62.69 Hordeum-LxHs-2H 54
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Map Data	Barley, Consensus 2007, SSR Barley, Consensus 2023, FHB Barley, LxHs
Species	Hordeum spontaneum Hordeum vulgare
Probe	HvXan
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remark	Locus in 'Barley, Consensus 2007, SSR' mapped in population(s) : Lina x H. spontaneum Insert contains repeat (CCT)6 Polymorphic Information Content (PIC) = 0.1 Developing laboratory = SCRI (R. Waugh)
Probe	HvXan
Locus	HvXan
Synonym	Synonym HAR35_1038 HAR35_29036
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
Remarks	Insert contains repeat (CCT)6 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.1
Type	PCR SSR
PCR primers	5' CAGCCACCTCCATAGTACTT 3' 5' CTGCTCTAGGCTCGTGTT 3' CAGCCACCTCCATAGTACTT CTGCTCTAGGCTCGTGTT
SSR size	140
Amplification Conditions	1 cycle of 3 min @ 94C, 1 min @ 58C, 1 min @ 72C, 30 cycles of 30 secs @ 94C, 30 secs @ 58C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Sequence	U26916
Source Species	Hordeum vulgare