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GrainGenes Marker Report: MWG2200

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Locus
MWG2200
Type
CAPS
RFLP
Chromosome
2H
Map
Barley-ConsensusFHB-2H154.41
[ Show all 7 ]
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Map Data
Barley, BinMap 2005
[ Show all 7 ]
Species
Hordeum vulgare
Probe
MWG2200
Reference
ReferenceVarshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091.
Data Source
Kleinhofs, Andris
Remark
This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin
Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb'
Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Igri x Franka
Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Igri x Franka
Locus in 'Barley, Consensus 2007, SSR' was a BIN marker in 'Barley, Consensus 2006, Marcel'


Probe
MWG2200
Locus
MWG2200
Reference
ReferenceMarcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500.
Remarks
Restriction Enzyme for CAPS marker is Ava I
PCR primers
5' agcacggcctccagcaccac 3'
5' agcacggcctccagcaccac 3'
5' atcctggagtacctcgaagccg 3'
5' atcctggagtacctcgaagccg 3'
STS primers
5' ATCCTGGAGTACCTCGAAGCCG
5' CGATGGACGTGCATCTAAGTCG
STS size
272
Amplification Conditions
5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 58 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence
AJ234832
MWG2200-5
Source Species
Hordeum vulgare