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GrainGenes Marker Report: MWG502

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Locus	MWG502
Type	CAPS RFLP STS
Synonym	Synonym MWG502
Chromosome	5H 7 (5H)
Chromosome Arm	5HS
Map	Barley-Consensus-5H -51.7 [ Show all 28 ]
	[ Show Nearby Loci ]
QTL with Locus Marker	MetaQ.DON_5H_1 MetaQ.FHB_5H_1 MetaQ.HT_5H_1 QFge.HaTR-5H.1 QGms.HaTR-5H
Map Data	Barley consensus [ Show all 28 ]
Species	Hordeum vulgare Hordeum vulgare subsp. spontaneum
Probe	MWG502
Reference	Sato K et al. (2009) A high-density transcript linkage map of barley derived from a single population. Heredity 103:110-117. [ Show all 6 ]
Data Source	Blake, Tom 05.04.19
Remark	Number of populations contributing to the placement of MWG502 in the Barley, Consensus 2006 DArT maps = 2 Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L9Oregon Wolfe Barleys x Vada,Steptoe x Morex,Igri x Franka Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec; Steptoe x Morex; Igri x Franka
Possible Orthologs	Xmwg502 Xmwg502-5R
Probe	MWG502
Locus	MWG502 Xmwg502 Xmwg502-5R
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500. Sayed-Tabatabaei BE et al. (1998) DNA sequencing and primer designing for RFLP clones evenly distributed in the barley genome. Barley Genetics Newsletter 28:15-18. Graner A et al. (1991) Construction of an RFLP map of barley Theoretical and Applied Genetics 83:250-256.
Remarks	Restriction Enzyme for CAPS marker is Msp I; Hpa II
Type	Genomic STS
PCR primers	5' tgtttagctaagctgttgctgagg 3' 5' ctctgaagcatggaaccagatttg 3'
STS primers	T7 side (5' to 3') ATCAAGATGAGGAAGCTGATGCAGT T3 side (5' to 3') TCTTGTCTGAAGCATGGAACCAGAT
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 55 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Sequence	AJ234471 AJ234470 MWG502 MWG502-5 MWG502-3
Linkage Group	5H
Insert Enzyme	PstI
Source Species	Hordeum vulgare
Insert Size	0.9