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GrainGenes Marker Report: PPO18

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Locus	PPO18
Type	Trait
Chromosome	2A
Chromosome Arm	2AL
Map	Wheat-Composite2004-2A 79
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	WMS312
Remark	Trait marker Xgwm312, Xgwm294 Nearby marker on Composite map: Xgwm312, Xgwm294b Position on Composite map estimated by GrainGenes.
Probe for Locus	WMS312
Locus	Xgwm312-2A [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)37 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATCGCATGATGCACGTAGAG 3' 5' ACATGCATGCCTACCTAATGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS312
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring