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GrainGenes Marker Report: Pm35

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Locus	Pm35
Type	Trait
Chromosome	5D
Chromosome Arm	5DL
Map	Wheat-Composite2004-5D 60
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	CFD26
Associated Gene	Pm35 (Triticum)
Gene Class	Reaction to Erysiphe graminis DC.
Reference	Miranda LM et al. (2007) Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 114:1451.
Remark	Trait marker Xcfd26-5D Nearby marker on Composite map: Xcfd26 Position on Composite map estimated by GrainGenes.
Probe for Locus	CFD26
Locus	Xcfd26-5D [ Show all 7 ]
Reference	Guyomarc'h H et al. (2002) Characterisation of polymorphic microsatellite markers from Aegilops tauschii and transferability to the D-genome of bread wheat Theoretical and Applied Genetics 104:1164-1172.
General Remarks	Quality Score = A Quality scores reflect the quality of amplification (from A very good quality to D multiple banding pattern : A and B can be used on capillary systems) Amplifies motif: (GAGAA)2(GA)37 Regularity of motif is perfect
Type	PCR SSR
PCR primers	Forward 5' TCAAGATCGTGCCAAATCAA 3' Reverse 5' ACTCCAAGCTGAGCACGTTT 3'
SSR size	271
Amplification Conditions	5 minutes at 94 degrees; 30 cycles (94 degrees for 30 seconds; 60 degrees for 30 seconds; 72 degrees for 30 seconds); 10 minutes at 72 degrees
Linkage Group	5D
Source Species	Aegilops tauschii