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GrainGenes Marker Report: Ppo-A1

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Gene	Ppo-A1
Gene Product	polyphenol oxidase
Sequence	EU371651 [ Show all 10 ]
Locus	Ppo-A1
Type	Trait
Chromosome	2A
Chromosome Arm	2AL
Map	Wheat-Composite2004-2A 80
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	PPO18 Sun D et al. (2005) A Novel STS Marker for Polyphenol Oxidase Activity in Bread Wheat Molecular Breeding 16:209-218. PPO33 He XY et al. (2007) Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theoretical and Applied Genetics 115:47. WMS312
Associated Gene	Ppo-A1 (Triticum)
Gene Class	Polyphenol oxidase
Reference	Sun D et al. (2005) A Novel STS Marker for Polyphenol Oxidase Activity in Bread Wheat Molecular Breeding 16:209-218. MAS Wheat. Functional Markers for processing quality genes in wheat MAS Wheat. Marker Assisted Selection in Wheat.
Remark	Trait marker Xgwm312-2A Nearby marker on Composite map: Xgwm312 Position on Composite map estimated by GrainGenes.
Probe for Locus	WMS312
Locus	Xgwm312-2A [ Show all 7 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)37 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATCGCATGATGCACGTAGAG 3' 5' ACATGCATGCCTACCTAATGG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS312
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring