GrainGenes Marker Report: Rf3
Locus
Rf3
Type
Gene Trait
Chromosome
1B
Chromosome Arm
1BS 1S
Map
Ta-Synthetic/Opata-1B 16.7 Triticeae-Sorrells-1 19 Triticum-Genes-1B -26.8 Wheat-Composite2004-1B 60
[ Show Nearby Loci ]
Map Data
Triticeae consensus Wheat genes Wheat, Synthetic x Opata [ GBrowser ]
2 Point Data
Gli-B1/rf3#1 Distance: 22.1 cM rf3/Glu-B1#1 Distance: 34.1 cM rf3/Nor-B1#1 Distance: 22.6 cM Rf3/Xbcd249
Species
Triticum aestivum
Probe
WMS131
Associated Gene
Rf3 (Triticum)
Gene Class
Restorers for cytoplasmic male sterility
Reference
Remark
For map 'Wheat, Synthetic x Opata', position approximated from mapping in another population. Trait marker Xgwm131-1BL Nearby marker on Composite map: Xgwm131.1 Position on Composite map estimated by GrainGenes.
Probe for Locus
WMS131
Locus
Rf3
[ Show all 12 ]
Reference
Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR SSR
PCR primers
5' AATCCCCACCGATTCTTCTC 3'
5' AGTTCGTGGGTCTCTGATGG 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS131
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring
GrainGenes Marker Report: Rf3
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