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GrainGenes Marker Report: Sr2

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Locus
Sr2
Type
Gene
Trait
Chromosome
3B
Chromosome Arm
3BS
Map
Triticum-Genes-3B-40.3
Wheat-2014-Consensus-Ug99-Update_3B7.68
Wheat-Composite2004-3B12
Wheat_Consensus_2014_Ug99_Rust_3B7.68
[ Show Nearby Loci ]
Map Data
Wheat genes
Wheat-2014-Consensus-Ug99-Update
Wheat_Consensus_2014_Ug99_Rust
Species
Triticum aestivum
Probe
csSr2
WMS533
Associated Gene
Sr2 (Triticum)
Gene Class
Reaction to Puccinia graminis Pers.
Reference
ReferenceYu L et al. (2014) A consensus map for Ug99 stem rust resistance loci in wheat. Theoretical and Applied Genetics 127:1561-1581.
ReferenceMago R et al. (2010) An accurate DNA marker assay for stem rust resistance gene Sr2 in wheat Theoretical and Applied Genetics 122:735-744.
ReferenceSpielmeyer W et al. (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.) Crop Science 43:333-336.
ReferenceSingh RP and McIntosh RA (1984) Complementary genes for resistance to Puccinia recondita tritici in Triticum aestivum II. Cytogenetic studies. Canadian Journal of Genetics and Cytology 26:736-742.
ReferenceSpielmeyer W et al. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr2 MAS Wheat. Marker Assisted Selection in Wheat.
Data Source
Matthews, David E.
Remark
Trait marker Xgwm533-3B.1
Nearby marker on Composite map: Xgwm533a
Position on Composite map estimated by GrainGenes.



Probe for Locus
WMS533
Locus
Xgwm533-3B
[ Show all 14 ]
Reference
ReferenceRoder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks
Insert contains repeat (CT)18(CA)20
PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type
PCR
SSR
PCR primers
5' AAGGCGAATCAAACGGAATA 3'
5' GTTGCTTTAGGGGAAAAGCC 3'
Amplification Conditions
3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism
WMS533
Source Species
Triticum aestivum
Source Germplasm
Chinese Spring