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GrainGenes Marker Report: Sr32

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Locus	Sr32
Chromosome	2D
Chromosome Arm	2DS
Map	Wheat-2014-Consensus-Ug99-Update_2A 0 Wheat-2014-Consensus-Ug99-Update_2B 0 Wheat-2014-Consensus-Ug99-Update_2D 0
	[ Show Nearby Loci ]
Map Data	Wheat-2014-Consensus-Ug99-Update
2 Point Data	Sr39/Sr32#1
Probe	csSr32-1 csSr32-2
Associated Gene	Sr32 (Triticum)
Gene Class	Reaction to Puccinia graminis Pers.
Reference	Mago R et al. (2013) Development of wheat–Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S-1 chromosome Theoretical and Applied Genetics 126:2943-2955. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Stem Rust Resistance. Sr32 MAS Wheat. Marker Assisted Selection in Wheat.
Remark	Sr32 has been mapped in 2A, 2B, and 2D.
Possible Orthologs	Sr32-2A Sr32-2B Sr32-2D
Probe for Locus	csSr32-1
Locus	Sr32
Reference	Mago R et al. (2013) Development of wheat–Aegilops speltoides recombinants and simple PCR-based markers for Sr32 and a new stem rust resistance gene on the 2S-1 chromosome Theoretical and Applied Genetics 126:2943-2955. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease resistance. Leaf Rust Resistance. Lr32 MAS Wheat. Marker Assisted Selection in Wheat.
Type	PCR
PCR primers	5'- GGT TTG GTG GCA ACT CAG GT -3' 5'- CAT AAG CCA AAG AGG CAC CA -3'
Amplification Conditions	Denaturing step: 95C, 2 min. Amplification step (30 cycles): 95C, 30 sec; 58C, 30 sec; 72C, 40 sec. Extension step: 72C, 5 min