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GrainGenes Marker Report: Tsn2

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Locus	Tsn2
Type	Trait
Chromosome	3B
Chromosome Arm	3BL
Map	Wheat-Composite2004-3B 55
	[ Show Nearby Loci ]
Species	Triticum aestivum
Probe	WMS285
Associated Gene	Tsn2 (Triticum)
Gene Class	Insensitivity to tan spot toxin
Reference	Singh PK et al. (2006) Identification and molecular mapping of a gene conferring resistance to Pyrenophora tritici-repentis race 3 in tetraploid wheat. Phytopathology 96:885-889.
Remark	Trait marker Xgwm285-3B Nearby marker on Composite map: Xgwm285b Position on Composite map estimated by GrainGenes.
Probe for Locus	WMS285
Locus	Xgwm285-3B [ Show all 6 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' ATGACCCTTCTGCCAAACAC 3' 5' ATCGACCGGGATCTAGCC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS285
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring