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GrainGenes Marker Report: WBE108

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Locus	WBE108
Type	CAPS
Chromosome	5H
Map	Hv-Consensus2006-Marcel-5H 11.99 Hv-Integrated2009-5H 15.1 Hv-L94/Vada-2006-5H 12.7
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Map Data	Barley, Consensus 2006, Marcel Barley, Integrated, Marcel 2009 Barley, L94 x Vada, 2006
Species	Hordeum vulgare
Probe	WBE108
Remark	Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = L94 x Vada Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : L94 x Vada; Oregon Wolfe Dom x Rec
Probe	WBE108
Locus	WBE108
Reference	Marcel TC et al. (2007) A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues. Theoretical and Applied Genetics 114:487-500.
Remarks	Restriction Enzyme for CAPS marker is HpyCH4 IV
PCR primers	5' aacaacaatggccgcctcgtcgtc 3' 5' ttgggctccggtggcgtgtgatac 3'
Amplification Conditions	5 min at 94 deg C; 35 cycles with 30 sec at 94 deg C, 30 sec at 62 deg C, and 1 min at 72 deg C; and a final extension step of 7 min at 72 deg C
Source Species	Hordeum vulgare