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GrainGenes Marker Report: WG622b

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Locus	WG622b
Type	RFLP
Chromosome	6H
Map	Barley-consensus-2003-6H 118.4
	[ Show Nearby Loci ]
Map Data	Barley consensus 2003
Probe	WG622
Data Source	Langridge, Peter J.
Possible Orthologs	WG622 [ Show all 9 ]
Probe for Locus	WG622
Locus	WG622 [ Show all 10 ]
Synonym	Synonym HAR35_12464 HAR35_28899 HAR35_43418
Type	Genomic STS
PCR primers	AGGTGGGACTGGAACCTGCATC CTCCTGTCAAACTGTTCCCTGA
STS primers	5' CTGCCTGTTGATTTTCCATG 5' TTCACCTTGCCATGACGA
STS size	161
Amplification Conditions	STS annealing temperature 58-60 oC
Sequence	BH854377 BH854376 WG622-5 WG622-3
Polymorphism	WG622 EcoRV
Linkage Group	[b 4S]
Insert Enzyme	PstI
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring
Insert Size	1.8