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GrainGenes Marker Report: WMC11

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Probe	WMC11
Locus	Xwmc11a-3A [ Show all 21 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1H4 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 184 bases located on 3A, 240 bases located on 3D AGR32. CT microstellite. Expected fragment 177 bases. Products obtained after PCR 61C anneal. (2loci?).
Type	PCR SSR
PCR primers	Forward TTgTgATccTggTTgTgTTgTgA at 95 length 23 bases, Tm 65 Reverse cAcccAgccgTTATATATgTTgA at 249 length 23 bases, Tm 62
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC11
Source Species	Triticum aestivum