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GrainGenes Marker Report: WMC126

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Probe	WMC126
Locus	Xwmc126-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7b9 Donor: Blake, Montana 7B9 Microsatellite : [GT]~60 [AC]~30 COMBINATION. Size of microsatellite only approximate. (CA)28 257 to 312, (GT)60 136 to 255.
Type	PCR SSR
PCR primers	Forward TTgTcAcAAAAgggTcgTAAAT at 48 length 22 bases, Tm 60 Reverse ATcTcgAgATgTcATcgAAAAT at 362 length 59 bases, Tm 59
SSR size	336 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide (nulls for 188 base allele).
Sequence	WMC126
Source Species	Triticum aestivum