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GrainGenes Marker Report: WMC14

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Probe	WMC14
Locus	Xwmc14-7D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1D5 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 239 bases located on 7D AGR36. Trimmed to Rsa1 fragment. ( CT) (CA) microsatellite. Expected fragment size=243. Microsatellite motif identical to 7a4, 8e11, 7f12, 11d10. Sequence matches second insert 6b7 (complement). PCR fragment obtained after 61C anneal.
Type	PCR SSR
PCR primers	Forward AcccgTcAccggTTTATggATg at 3 length 22 bases, Tm 69 Reverse TccAcTTcAAgATggAgggcAg at 224 length 22 bases, Tm 68
SSR size	243 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC14
Source Species	Triticum aestivum