Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: WMC144

[Submit comment/correction]

Probe	WMC144
Locus	Xwmc144-2D wmc144
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5g10 Donor: Henry, Lismore Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 144 bases located on 2D (CA)14 69 to 96. Bases 70-247 (complement) ie 3' side of microsatellite match 1b4, 5f2.
Type	PCR SSR
PCR primers	Forward ggAcAccAATccAAcATgAAcA at 7 length 22 bases, Tm 65 Reverse AAggATAgTTgggTggTgcTgA at 128 length 22 bases, Tm 65
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC144
Source Species	Triticum aestivum