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GrainGenes Marker Report: WMC149

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Probe	WMC149
Locus	Xwmc149a-5B [ Show all 17 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h1 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 5B, 185 bases located on 1B, 230 bases located on 2B AGR204.8.SEQ. Trimmed to Rsa1 at both ends. (CT)8 50 to 65, (CT)24 68 to 115. (3-4 loci, 2-3 polymorphic).
Type	PCR SSR
PCR primers	Forward AcAgAcTTggTTggTgccgAgc at 6 length 22 bases, Tm 70 Reverse ATgggcgggggTgTAgAgTTTg at 214 length 22 bases, Tm 70
SSR size	230 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC149
Source Species	Triticum aestivum