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GrainGenes Marker Report: WMC152

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Probe	WMC152
Locus	Xwmc152-6B wmc152 wmc152-6B wmc152-6D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11d3 Donor: Rogers, Monsanto AGR204.20.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)19 166 to 203, (GT)20 96 to 135, (GT)13 140 to 165.
Type	PCR SSR
PCR primers	Forward cTATTggcAATcTAccAAAcTg at 7 length 22 bases, Tm 58 Reverse TcTcTTcTTgccAcATATTcgT at 236 length 22 bases, Tm 61
SSR size	251 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC152
Source Species	Triticum aestivum