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GrainGenes Marker Report: WMC158

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Probe	WMC158
Locus	Xwmc158-7A Xwmc158
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b6 Donor: Rogers, Monsanto Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 369 bases located on 7A AGR204.42.SEQ. Trimmed to insert at 5' end and to Rsa1 at 3' end. (GT)17 287 to 320, (CT)24 119 to 166, (CT)9 197 to 214, (CT)9 241 to 258.
Type	PCR SSR
PCR primers	Forward AAcTggcATcATgTTTTgTAgg at 27 length 22 bases, Tm 61 Reverse AATgTAgTcAAAAgAggTggTg at 382 length 22 bases, Tm 58
SSR size	377 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC158
Source Species	Triticum aestivum