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GrainGenes Marker Report: WMC168

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Probe	WMC168
Locus	Xwmc168-7A Xwmc168 wmc168
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9d2 Donor: Bernard, Clermont-Ferrand Trimmed to Rsa1 site at 5' end, and to insert at 3' end. (CT)27 338 to 391. Big difference between Tms of primers.
Type	PCR SSR
PCR primers	Forward AAcAcAAAAgATccAAcgAcAc at 91 length 22 bases, Tm 63 Reverse cAgTATAgAAggATTTTgAgAg at 388 length 22 bases, Tm 52
SSR size	319 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC168
Source Species	Triticum aestivum