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GrainGenes Marker Report: WMC170

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Probe	WMC170
Locus	Xwmc170b-2A Xwmc170a-2D Xwmc170-2A Xwmc170
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9h2 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 227 bases located on 2A Trimmed to Rsa1 at both ends. Multiple inserts in this clone joined by linkers at 258-275. (CA)19 378 to 415. Primers are to second insert.
Type	PCR SSR
PCR primers	Forward AcATccAcgTTTATgTTgTTgc at 331 length 22 bases, Tm 61 Reverse TTggTTgcTcAAcgTTTAcTTc at 539 length 22 bases, Tm 61
SSR size	230 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC170
Source Species	Triticum aestivum