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GrainGenes Marker Report: WMC18

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Probe	WMC18
Locus	Xwmc18-2D Xwmc18 wmc18-2D wmc18 Xwmc018
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1D6 Donor: Nickerson Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 237 bases located on 2D AGR44. (CA)(CT) compound microsatellite. Expected fragment size 230.
Type	PCR SSR
PCR primers	Forward cTggggcTTggATcAcgTcATT at 49 length 22 bases, Tm 69 Reverse AgccATggAcATggTgTccTTc at 258 length 22 bases, Tm 67
SSR size	230 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide;
Sequence	WMC18
Source Species	Triticum aestivum