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GrainGenes Marker Report: WMC182

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Locus	wmc182
Type	microsatellite
Map	Durum Wheat, NSGC Lr 6B 84.9 [ Show all 9 ]
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat AB, Carotenoid QTL, 2017 Wheat, Yr genes and QTL
Probe	WMC182
Possible Orthologs	Xwmc182 [ Show all 18 ]
Probe	WMC182
Locus	Xwmc182-7A [ Show all 19 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9g6 Donor: Bernard, Clermont-Ferrand Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 165 bases located on 7B Trimmed to Rsa1 at both ends. (CT)16 194 to 225.
Type	PCR SSR
PCR primers	Forward gTATcTcAcgAgcATAAcAcAA at 91 length 22 bases, Tm 57 Reverse gAAAgTgTATggATcATTAggc at 228 length 22 bases, Tm 57
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC182
Source Species	Triticum aestivum