Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: WMC183

[Submit comment/correction]

Probe	WMC183
Locus	Xwmc183 Xwmc183-1A wmc183 wmc183-1A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 9h6 Donor: Bernard, Clermont-Ferrand Trimmed to insert at 5' and and to Rsa1 at 3' end. Bases 266-347 (complement) ie. 3' side of microsatellite match part of 11g3. Bases 1-290 (ie 5' side of microsatellite) match part of 3c12. (CA)18 243 to 278.
Type	PCR SSR
PCR primers	Forward cAgAAAcggcTcAAcTTAAcAA at 95 length 22 bases, Tm 61 Reverse TcTgATcTcgTgATcAgAATAg at 297 length 22 bases, Tm 56
SSR size	224 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic on acrylamide.; 51C anneal, smear;
Sequence	WMC183
Source Species	Triticum aestivum