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GrainGenes Marker Report: WMC198

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Probe	WMC198
Locus	Xwmc198 Yr32
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4d11 Donor: Morell, Canberra Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)27 67 to 120.
Type	PCR SSR
PCR primers	Forward cAcgcTgccATcAcTTTTAc at 27 length 20 bases, Tm 61 Reverse TTgAAgTggTcATTgTTgcT at 166 length 20 bases, Tm 59
SSR size	159 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC198
Source Species	Triticum aestivum