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GrainGenes Marker Report: WMC201

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Probe	WMC201
Locus	Xwmc201-6A Xwmc201 wmc201-6A wmc201
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4f12 Donor: Morell, Canberra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 243 bases located on 6A Trimmed to insert at both ends (remving mostly Ns). (CA)25 201 to 250. Bases 162 to 249 show homology to 26f10 bases 402 to 489 (88 in longest run, maxScore = 114).
Type	PCR SSR
PCR primers	Forward cATgcTcTTTcAcTTgggTTcg at 85 length 22 bases, Tm 66 Reverse gcgcTTgcAggAATTcAAcAcT at 312 length 22 bases, Tm 67
SSR size	249 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC201
Source Species	Triticum aestivum