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GrainGenes Marker Report: WMC233

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Probe	WMC233
Locus	Xwmc233-5D Xwmc233 wmc233
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5b1.2 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 262 bases located on 5D Trimmed to Rsa1 at both ends. Multiple inserts (2) joined by linker related sequence 310-317.(CA)11 367 to 388, (CA)14 391 to 418, (GT)23 56 to 101. Primers shown are for second insert.
Type	PCR SSR
PCR primers	Forward gAcgTcAAgAATcTTcgTcggA at 318 length 22 bases, Tm 66 Reverse ATcTgcTgAgcAgATcgTggTT at 556 length 22 bases, Tm 65
SSR size	260 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC233
Source Species	Triticum aestivum