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GrainGenes Marker Report: WMC237

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Probe	WMC237
Locus	Xwmc237
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5e2 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 130 bases located on 7B, 90 bases located on 7B Trimmed to Rsa1 at 5' end and to insert at 3' end. (TG)6(TC)1(TG)6(TT)1(TG)6 at 119-158.
Type	PCR SSR
PCR primers	Forward TAAgTgAATcgATgggcgTggA at 97 length 22 bases, Tm 68 Reverse cAgTgTgcAggggAAgcAgTgT at 200 length 22 bases, Tm 69
SSR size	125 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC237
Source Species	Triticum aestivum