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GrainGenes Marker Report: WMC247

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Probe	WMC247
Locus	Xwmc247-5B Xwmc247
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5d5.2 Donor: Sharp, Camden Derived from full sequence - this is the second insert only, without linkers. Primers shown are for this insert insert. (GT)13 107-132.
Type	PCR SSR
PCR primers	Forward cATgTTgATAAccgAcTcgcAg at 80 length 22 bases, Tm 65 Reverse TgAAAcTAggggccAcgAATAA at 137 length 22 bases, Tm 64
SSR size	79 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC247
Source Species	Triticum aestivum