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GrainGenes Marker Report: WMC25

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Probe	WMC25
Locus	Xwmc25b-2B [ Show all 19 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 2c9 Donor: Wang, Utah (GT)26 193 to 244. >2 Polymorphic loci.
Type	PCR SSR
PCR primers	Forward TcTggccAggATcAATATTAcT at 102 length 22 bases, Tm 59.0 Reverse TAAgATAcATAgATccAAcAcc at 246 length 22 bases, Tm 53.0
SSR size	166 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC25
Source Species	Triticum aestivum