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GrainGenes Marker Report: WMC256

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Probe	WMC256
Locus	Xwmc256-6A Xwmc256 wmc256-6A wmc256
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4h2.1 Donor: Gupta, Meerut Small linker like sequence at 234-241 in original ie. multiple inserts (2) in this sequence, this record shows first insert only (up to but excluding linker). No trimming done to sequence at 5'. (CA)12 65 to 88.
Type	PCR SSR
PCR primers	Forward ccAAATcTTcgAAcAAgAAccc at 37 length 22 bases, Tm 64 Reverse AccgATcgATggTgTATAcTgA at 132 length 22 bases, Tm 61
SSR size	117 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC256
Source Species	Triticum aestivum