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GrainGenes Marker Report: WMC257

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Probe	WMC257
Locus	Xwmc257-2B Xwmc257 wmc257 wmc257-2B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4c3 Donor: Gupta, Meerut Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 331 bases located on 2B Trimmed to Rsa1 at 3' end. (GA)14 85 to 112.
Type	PCR SSR
PCR primers	Forward ggcTAcAcATgcATAccTcT at 20 length 20 bases, Tm 57 Reverse cgTAgTgggTgAATTTcggA at 329 length 20 bases, Tm 63
SSR size	329 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC257
Source Species	Triticum aestivum