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GrainGenes Marker Report: WMC264

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Probe	WMC264
Locus	Xwmc264-3A [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4c6 Donor: Gupta, Meerut No trimming done to sequence. (GA)16 81 to 112.
Type	PCR SSR
PCR primers	Forward cTccATcTATTgAgcgAAggTT at 55 length 22 bases, Tm 61 Reverse cAAgATgAAgcTcATgcAAgTg at 166 length 22 bases, Tm 63
SSR size	133 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC264
Source Species	Triticum aestivum